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KMID : 0545120020120040664
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 4 p.664 ~ p.668
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cDNA Microarray Analysis of Transcriptional Response to Hyperin in Human Gastric Cancer Cells
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Jeoung, Doo Il/Jeoung, Doo Il
Kim, Jae Hwan/Lee, Youn Hyung/Baek, Myung In/Lee, Seong Eun/Baek, Nam In/Kim, Hae Yeong/Kim, Jae Hwan/Lee, Youn Hyung/Baek, Myung In/Lee, Seong Eun/Baek, Nam In/Kim, Hae Yeong
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Abstract
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Antioxidants either scavenge superoxide and free radicals or stimulate the detoxification mechanisms within cells, resulting in increased detoxification of free radicals formation. Hyperin, isolated from the stem of Uncaria rhynchophylla, prevented oxygen radical formation and inhibited lipid oxidation. The effective concentrations were 31.3μM for a radical scavenging assay and 2.2μM for a microsome assay, cDNA microarray analysis to determine which genes were modulated by hyperin found that 50 genes were upregulated and 37 genes were downregulated in SNU-668 human gastric cancer cells. Among these genes, thirteen genes that were significantly affected by hyperin were verified by RT-PCR for their effect of genetic reprogramming.
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